High-resolution cryo-EM of macromolecular assemblies


Our research is focused on structure and function of macromolecular assemblies using three-dimensional cryo-electron microscopy (cryoEM), combined with biochemical, biophysical and molecular biology methods. CryoEM is a powerful tool for structure determination of large protein complexes and macromolecular assemblies, and their conformational changes to provide structural snapshots along dynamic processes. Research efforts in our lab are currently directed to two areas in biology: (I) HIV pathogenesis, particularly HIV capsid assembly, maturation, and interactions with host cell factors; (II) molecular mechanisms of signal transduction in bacterial chemotaxis. We are also developing technologies to bridge the gap in single molecule imaging, by integrating optical and electron imaging methods (correlative microscopy), and working at the interface of nanotechnology and biology.


Education

PhD 1998, University of Virginia, Molecular Biophysics

Postdoctoral Training

1998-2000 National Institute of Health, NIDDK
2000-2002 National Institute of Health, NCI


 

Department of Structural Biology
University of Pittsburgh
3501 5th Avenue
Pittsburgh, PA 15260

Phone: (412) 383-5907
Fax: (412) 648-8998

E-mail: pez7@pitt.edu

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