My current research seeks to gain structural information of the parathyroid hormone receptor (PTHR), a membrane protein critical to bone and kidney health. PTHR is a G protein-coupled receptor. Multiple endogenous ligands bind to PTHR, and each ligand has unique signaling properties. To understand the mechanisms of ligand binding and PTHR activation, I aim to solve high-resolution structures of PTHR and PTHR-containing complexes using X-ray crystallography and electron microscopy.
Biology BA, Truman State University, 2014
PhD Advisor: Dr. Jean-Pierre Vilardaga
University of Pittsburgh School of Medicine
Dept. Pharmacology & Chemical Biology
W1301 Biomedical Science Tower, 200 Lothrop Street
Pittsburgh, PA 15261
email: ljc32 [at] pitt.edu
- Stevenson HP, Lin G, Barnes CO, Sutkeviciute I, Krzysiak T, Weiss SC, Reynolds S, Wu Y, Natarajan V, Makhov AM, Lawrence R, Lamm E, Clark L, Gardella TJ, Hogue BG, Ogata CM, Ahn J, Gronenborn AM, Conway JF, Vilardaga JP, Cohen AE, Calero G. (2016) Transmission electron microscopy for the evaluation and optimization of crystal growth. Acta Crystallogr D Struct Biol. 72(Pt 5):603-15
- Xiao K, Zhao Y, Choi M, Liu H, Blanc A, Qian J, Xiao Y, Clark L, Li S. Revealing the architecture of protein complexes by an orthogonal approach combining HDXMS, CXMS, and disulfide Trapping. Nat Protoc. 2018 Jun;13(6):1403-1428.
- White AD, Fang F, Jean-Alphonse FG, Clark L et al. Ca2+ allostery in PTH-receptor signaling. Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3294-3299.
- Zhao L-H, Ma S-S, Sutkeviciute I, Shen D-D, Zhou XE, de Waal PW, Li C-Y, Kang Y, Clark L et al. Structure and dynamics of parathyroid hormone receptor-1. Science. 2019 Apr 12;364(6436):148-153.