From Targets to Therapies
Check out a special issue in the ACS Medicinal Chemistry Letters. Peter Wipf in MBSB co-edited this issue. Prof. Wipf commented “This year, ACS Medicinal Chemistry Letters celebrates its 10th anniversary. To recognize this occasion, we are publishing a Special Issue entitled “Medicinal Chemistry: From Targets to Therapies.” In 2020, the coronavirus SARS-CoV has overtaken the world amidst a global pandemic. Consequently, antiviral research, and vaccine development and production, have been catapulted into the spotlight. In the face of the problem of bacterial resistance, there is a pressing need for enhanced, sustained research on anti-infective agents. At the same time, pharmaceutical treatments in many other fields are still urgently needed. Medicinal Chemistry is called upon to answer gaps and inequalities in addressing public health needs, more than ever on a moment’s notice.”
A beautiful night.
“Another beautiful night at #carnegiemellon”
From Prof. Dahl in MBSB
A student comment - Dahl’s laboratory
Doing good science starts with learning how to collect data properly, beginning with your controls. The better your baseline, the easier it is to show a definitive result. In this case, it enabled us to accurately quantify dissociation of cell-cell contacts after drug treatment.
Isothermal Titration Calorimetry (ITC)
This is Ananya from the Hinck lab. She is working on ITC to assess protein-protein binding interactions.
Blinded by the light
Blinded by the light: Activating gene editing in fish with light
Third-year MBSB student Wes Brown and Wenyuan Zhou, a fellow graduate student in the Alex Deiters lab have recently published their work on photocaged gRNA and its application for optical control of CRISPR-Cas9 gene editing in mammalian cells and zebrafish embryos: W. Zhou, W. Brown, A. Bardhan, M. Delaney, A. S. Ilk, R. R. Rauen, S. I. Kahn, M. Tsang, A. Deiters, Angew. Chem. Int. Ed. 2020, 59, 8998.
The Deiters group installed photolabile “cages” in the protospacer region of the gRNA that block target DNA binding until “decaged” with 405 nm light. Controlling CRISPR-Cas9 function by installing caging groups in the gRNA has become a hot field, with several independent groups publishing on the topic recently. Optical control allows for spatial and temporal restriction of gene editing that can be used to reduce off-target editing and to study gene networks in a developing animal. They are also working on applying this technology to other CRISPR-based tools to develop optical control of base editing and RNA editing.